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Quantitative Real Time PCR Methods and Protocols 1st edition by Roberto Biassoni, Alessandro Raso ISBN 1493946655 9781493946655

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ISBN 10: 1493946655
ISBN 13: 9781493946655
Author: Roberto Biassoni, Alessandro Raso

Quantitative Real-Time PCR: Methods and Prools focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory prools and tips on troubleshooting and avoiding known pitfalls.

 

Practical and authoritative, Quantitative Real-Time PCR: Methods and Prools aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.

Quantitative Real Time PCR Methods and Protocols 1st Table of contents:

Part I. Foundations and Technical Advances in qPCR

  1. Twenty Years of qPCR: A Mature Technology?

  2. Minimum Information Necessary for Quantitative Real-Time PCR Experiments

  3. Selection of Reliable Reference Genes for RT-qPCR Analysis

  4. Introduction to Digital PCR

  5. Mediator Probe PCR: Detection of Real-Time PCR by Label-Free Probes and a Universal Fluorogenic Reporter

Part II. Nucleic Acid Integrity and Quantification

  1. mRNA and microRNA Purity and Integrity: The Key to Success in Expression Profiling

  2. Absolute Quantification of Viral DNA: The Quest for Perfection

Part III. Infectious Disease Diagnostics

  1. A Multiplex Real-Time PCR Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical Samples

  2. Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of Community-Acquired Pneumonia

  3. A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Conditions

Part IV. Oncology and Hematology Applications

  1. Real-Time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia

  2. A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR

  3. Circulating Cell-Free DNA in Cancer

Part V. Applications in Transplantation and Gene Expression

  1. Gene Expression Analysis by qPCR in Clinical Kidney Transplantation

  2. Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and microRNA Data

Part VI. Clinical and Diagnostic Innovations Using Digital PCR

  1. Clinical Applications Using Digital PCR

  2. Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCR

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Tags: Roberto Biassoni, Alessandro Raso, Real Time, Methods and Protocols