Instant download Culture of Animal Cells A Manual of Basic Technique and Specialized Applications 6e pdf, docx, kindle format all chapters after payment.
Product details:
- ISBN 13: 9780470528129
- Author: R. Ian Freshney
This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage
Table contents:
1. Introduction.
2. Biology of Cultured Cells.
3. Laboratory Design, Layout, and Equipment.
4. Equipment and Materials.
5. Aseptic Technique.
6. Safety, Bioethics, and Validation.
7. Culture Vessels and Substrates.
8. Defined Media and Supplements.
9. Serum-Free Media.
10. Preparation and Sterilization.
11. Primary Culture.
12. Subculture and Cell Lines.
13. Cloning and Selection.
14. Cell Separation.
15. Characterization.
16. Differentiation.
17. Transformation and Immortalization.
18. Contamination.
19. Cryopreservation.
20. Quantitation.
21. Cytotoxicity.
22. Specialized Cells.
23. Stem Cells, Germ Cells, and Amniocytes.
24. Culture of Tumor Cells.
25. Three-Dimensional Culture.
26. Scale-up and Automation.
27. Specialized Techniques.
28. Training Programs.
29. Problem Solving.
30. In Conclusion.
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