Essentials in Modern HPLC Separations 1st edition by Serban Moldoveanu, Victor David – Ebook PDF Instant Download/Delivery: 0123850140, 9780123850140
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ISBN 10: 0123850140
ISBN 13: 9780123850140
Author: Serban Moldoveanu, Victor David
Essentials in Modern HPLC Separations discusses the role of separation in high performance liquid chromatography (HPLC). This up-to-date reference systematically covers new developments in types and characteristics of stationary phases, mobile phases, and other factors of this technique that influence separation of compounds being analyzed. The volume also considers the selection process for stationary and mobile phases in relation to the molecules being separated and examined, as well as their matrices.
The book includes a section on the contemporary applications of HPLC, particularly the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, and more.
- Discusses key parameters in HPLC separation
- Describes interrelation between various HPLC features (solvent pressure, separation, detection)
- Includes a large number of references
Essentials in Modern HPLC Separations 1st Table of contents:
Chapter 1. Basic Information about HPLC
1.1 Introduction to HPLC
1.2 Main Types of HPLC
1.3 Practice of HPLC
1.4 Overview of HPLC Instrumentation
References
Chapter 2. Parameters that Characterize HPLC Analysis
2.1 Parameters Related to HPLC Separation
2.2 Experimental Peak Characteristics in HPLC
References
Chapter 3. Equilibrium Types in HPLC
3.1 Partition Equilibrium
3.2 Adsorption Equilibrium
3.3 Equilibria Involving Ions
3.4 Equilibrium in Size-Exclusion Processes
3.5 The Influence of PH on Retention Equilibria
3.6 The Influence of Temperature on Retention Equilibria
References
Chapter 4. Intermolecular Interactions
4.1 Forces Between Ions and Molecules
4.2 Forces Between Molecules and a Surface
References
Chapter 5. Retention Mechanisms in Different HPLC Types
5.1 Retention in Reversed-Phase Chromatography
5.2 Retention and Separation Process in Ion-Pair Chromatograpy
5.3 Retention and Separation on Polar Stationary Phases
5.4 Retention Process in Ion-Exchange Chromatography
5.5 Separation Process in Chiral Chromatography
5.6 Retention Process in Size-Exclusion Chromatography
5.7 Retention Process in Other Chromatography Types
References
Chapter 6. Stationary Phases and Their Performance
6.1 Solid Supports for Stationary Phases
6.2 Reactions Used for Obtaining Active Groups of Stationary Phases
6.3 Properties of Stationary Phases and Columns
6.4 Hydrophobic Stationary Phases and Columns
6.5 Polar Stationary Phases and Columns
6.6 Stationary Phases and Columns for Ion-Exchange, Ion-Moderated, and Ligand-Exchange Chromatography
6.7 Stationary Phases and Columns for Chiral Chromatography
6.8 Stationary Phases and Columns for Size-Exclusion Chromatography
6.9 Stationary Phases and Columns in Immunoaffinity Chromatography
References
Chapter 7. Mobile Phases and Their Properties
7.1 Characterization of Liquids as Solvents
7.2 Additional Properties of Liquids Affecting Separation
7.3 Properties of the Mobile Phase of Importance in HPLC, not Related to Separation
7.4 Buffers and other Additives in HPLC
7.5 Gradient Elution
7.6 Mobile Phase in Reversed-Phase Chromatography
7.7 Mobile Phase in Ion-Pair Liquid Chromatography
7.8 Mobile Phase in HILIC and NPC
7.9 Mobile Phase in Ion-Exchange and Ion-Moderated Chromatography
7.10 Mobile Phase in Chiral Chromatography
7.11 Mobile Phase for Size-Exclusion Separations
References
Chapter 8. Solutes in HPLC
8.1 Nature of the Solute
8.2 Parameters for Solute Characterization in the Separation Process
8.3 Other Parameters for Solute Characterization
References
Chapter 9. HPLC Analysis
9.1 Chemical Nature of the Analytes and the Choice of HPLC Type
9.2 The Quantity of Sample Injected for HPLC Analysis
9.3 Estimation of Parameters Describing the Separation
9.4 Steps in Development and Implementation of an HPLC Separation
9.5 Separations by RP-HPLC
9.6 Separations by Ion-Pair Chromatography
9.7 Separations by HILIC and NPC
9.8 Separations by Ion-Exchange Chromatography
9.9 Chiral Separations
9.10 Separations by Size-Exclusion Chromatography
References
Symbols
Index
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