Neural Stem Cell Assays 1st Edition by Navjot Kaur, Mohan C. Vemuri – Ebook PDF Instant Download/Delivery: 1118308264, 9781118308264
Full download Neural Stem Cell Assays 1st Edition after payment
Product details:
ISBN 10: 1118308264
ISBN 13: 9781118308264
Author: Navjot Kaur, Mohan C. Vemuri
Neural stem cells offer a valuable model system for delineating the cellular and developmental processes in normal and diseased states of the central nervous system. In particular, neural stem cells have huge potential in regenerative medicine, owing to their expansion capability in culture and the ability to differentiate into multiple sub-neural lineages. Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures. Including an overview of progress in the field over the past decade, Neural Stem Cell Assays is a one-stop reference for consistent methods and reliable tools that span the entire assay work flow, from isolation or generation of neural stem cells to characterization, manipulation and final application of neural stem cells in disease paradigms such as Parkinson’s disease, multiple sclerosis and amyotrophic lateral sclerosis. An excellent source of information for academic, pharmaceutical and biotechnology researchers who are new to the neural stem cell field, Neural Stem Cell Assays is an invaluable to experienced users who wish to integrate newly developed tools and technologies into their workflow. The book also covers important course material for students at the undergraduate and graduate level who are learning the basics of neural stem cell cultures, and differentiation to sub-neural lineages.
Neural Stem Cell Assays 1st Table of contents:
Chapter 1: Neural Differentiation of Pluripotent Stem Cells
1.1 Introduction
1.2 NSC Derivation from Rosette Formation of Embryoid Bodies
1.3 Rosette Free NSC in a Monolayer Culture
1.4 Pathways Involved in Neural Tube and Neural Crest Lineages
1.5 Differentiation and Gene Expression in Human Brain Development
1.6 Conclusions
References
Chapter 2: The History and Design of Assays for the Identification and Characterization of Neural Stem Cells
2.1 Introduction
2.2 Materials
2.3 Methods
2.4 Notes
Acknowledgments
References
Chapter 3: Culture of Human Neural Stem Cells
3.1 Introduction
3.2 Materials
3.3 Methods
3.4 Notes
3.5 Expected Results
References
Chapter 4: GMP4-Compatible Production and Expansion of Human Neural Stem Cells
4.1 Introduction
4.2 Materials
4.3 Methods
4.4 Notes
Acknowledgments
References
Chapter 5: Primary Rat Neural Cultures
5.1 Introduction
5.2 Materials
5.3 Methods
5.4 Notes
References
Chapter 6: Cryopreservation of Human Neural Stem and Progenitor Cells
6.1 Introduction
6.2 Materials
6.3 Methods
6.4 Notes
References
Chapter 7: Cryopreservation and Recovery of Primary Rat Neural Cells
7.1 Introduction
7.2 Materials
7.3 Methods
7.4 Notes
References
Chapter 8: Efficient Induction and Scale up Generation of Neural Stem Cells from Human Pluripotent Stem Cells
8.1 Introduction
8.2 Materials
8.3 Methods
8.4 Notes
References
Chapter 9: Directed Differentiation of Human NSC/NPC into Dopaminergic Neurons
9.1 Introduction
9.2 Materials
9.3 Methods
9.4 Notes
References
Chapter 10: In vitro Differentiation of Pluripotent Stem Cells towards either Forebrain GABAergic or Midbrain Dopaminergic Neurons
10.1 Introduction
10.2 Materials
10.3 Methods
10.4 Notes
10.5 Typical Results
References
Chapter 11: Generation of Astrocytes from Human Pluripotent Stem Cells using a Defined System
11.1 Introduction
11.2 Materials and Methods
11.3 Notes
References
Chapter 12: Directed Differentiation of Human PSC into Oligodendrocytes
12.1 Introduction
12.2 Materials
12.3 Methods
12.4 Notes
Acknowledgments
References
Chapter 13: Directed Differentiation towards Human Neural Retinal Cells
13.1 Introduction
13.2 Materials
13.3 Methods
References
Chapter 14: Directed Differentiation of Photoreceptors and Retinal Pigment Epithelium from Adult Mouse Retinal Stem Cells
14.1 Introduction
14.2 Materials
14.3 Methods
14.4 Notes
Acknowledgments
References
Chapter 15: Induction of Schwann Cells from Rat Bone Marrow Mesenchymal Stem Cells
15.1 Introduction
15.2 Materials
15.3 Method
15.4 Notes
References
Chapter 16: Neural Cell Viability Assays
16.1 Introduction
16.2 Materials
16.3 Methods
16.4 Notes
References
Chapter 17: Genetic Modification of Pluripotent Stem Cell Derived Human Neural Progenitor Cells
17.1 Introduction
17.2 Materials
17.3 Methods
17.4 Notes
Acknowledgments
References
Chapter 18: Electrophysiology of Neurons
18.1 Introduction
18.2 Materials
18.3 Methods
18.4 Notes
References
Chapter 19: Induction and Transplantation of Neural Stem Cells from Human Pluripotent Stem Cells in Experimental Models (Rat) of Parkinson’s disease
19.1 Introduction
19.2 Materials
19.3 Methods
19.4 Notes
Acknowledgments
References
Chapter 20: Transplantation of Human Neural Stem Cells and Progenitors in Animal Models of Disease
20.1 Introduction
20.2 General Principles of Effective Transplantation
20.3 Different Methods of Transplantation
20.4 Materials and Method
20.5 Conclusions
20.6 Notes
Acknowledgments
References
Chapter 21: Morphometric Assays for Neural Differentiation and Synaptic Development of Human iPSC-Derived NSC
21.1 Introduction
21.2 Materials
21.3 Method
21.4 Notes
Acknowledgments
References
Chapter 22: BacMam Transduction of Neural Stem Cells
22.1 Introduction
22.2 Materials
22.3 Methods
22.4 Notes
Acknowledgments
References
Chapter 23: The Proximity Ligation Assay: A High Throughput Technique for Protein Analysis in Neuroscience
23.1 Introduction
23.2 Materials
23.3 Methods
23.4 Notes
23.5 Troubleshooting
Acknowledgments
References
Chapter 24: Quantification of Micro-RNA Expression in Human Neural Stem Cells using TaqMan Assays
24.1 Introduction
24.2 Materials
24.3 Methods
24.4 Notes
References
Chapter 25: Genetic Reporter Cell Lines: Tools for Stem Cell Biology and Drug Discovery
25.1 Pluripotent Stem Cell Reporter Lines
25.2 Choice of PSC Genetic Reporter Systems
25.3 Reporter Systems
25.4 Multiple Reporter Lines
25.5 Use of Genetic Reporter Escs to Study Neural Differentiation
People also search for Neural Stem Cell Assays 1st:
neural stem cells review
stem cell neural differentiation
neural stem cell differentiation protocol
neural stem cell function
mouse neural stem cells
Tags: Neural Stem, Cell Assays, Navjot Kaur, Mohan Vemuri