Optogenetics Methods and Protocols 1st edition by Arash Kianianmomeni – Ebook PDF Instant Download/Delivery: 1493980629, 9781493980628
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ISBN 10: 1493980629
ISBN 13: 9781493980628
Author: Arash Kianianmomeni
This volume provides researchers with up-to-date prools and takes a close look at current research and promising applications. Optogenetics: Methods and Prools contains a collection of recently developed technical prools on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Prools, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications.
Optogenetics Methods and Protocols 1st Table of contents:
Optogenetics: Basic Concepts and Their Development
Chapter 1: Natural Resources for Optogenetic Tools
Chapter 2: Algal Photobiology: A Rich Source of Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics
Chapter 3: Reversible Photoregulation of Gene Expression and Translation
Chapter 4: Controlling Protein Activity and Degradation Using Blue-Light
Chapter 5: Photo-Control of Protein Function Using Photoactive Yellow Protein
Chapter 6: A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators
Chapter 7: Optogenetic Control of Pancreatic Islets
Chapter 8: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Chapter 9: Perfect Channelrhodopsins: A User Guide
Chapter 10: Optogenetics in Drosophila Neuroscience
Chapter 11: Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
Chapter 12: Optogenetic Modulation of Locomotor Activity on Free Behaving Rats
Chapter 13: Combining Optogenetic and Chemogenetic Control of Neurons
Chapter 14: Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
Chapter 15: An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
Chapter 16: Optogenetic Approaches for Mesoscopic Brain Mapping
Chapter 17: Optogenetic Tools for Confined Stimulation in Deep Brain Structures
Chapter 18: Remote Patterning of Transgene Expression Using Near Infrared-Responsive Plasmonic Hydrogels
Chapter 19: Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias
Chapter 20: Inscribing Opal Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts
Chapter 21: Optogenetic Engineering of Atrial Cardiomyocytes
Chapter 22: A Multi-Channel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments
Chapter 23: Optogenetic Control of Fibroblast Growth Factor Receptor Signaling
Chapter 24: Protein Inactivation by Optogenetic Trapping in Living Cells
Chapter 25: Optogenetic Manipulation of Selective Neural Activity in Free-Moving Drosophila Adults
Chapter 26: Guidelines for Photoreceptor Engineering
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Arash Kianianmomeni,Optogenetics Methods,Protocols