Practical Manual of In Vitro Fertilization 1st edition by Zsolt Peter Nagy, Alex Varghese, Ashok Agarwal – Ebook PDF Instant Download/Delivery: 1441917802, 9781441917805
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ISBN 10: 1441917802
ISBN 13: 9781441917805
Author: Zsolt Peter Nagy, Alex Varghese, Ashok Agarwal
The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future. An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.
Practical Manual of In Vitro Fertilization 1st Table of contents:
1. Journey of Human Gametes In Vitro: 1978–2010
1. Setting Up and Running an IVF Laboratory
2. Building the Laboratory
3. Air Quality Management
4. Organizational Aspects of the Laboratory in a Tertiary Care ART Center
5. Quality Control Management
6. Daily, Weekly, and Regular Preparations for the IVF Laboratory
7. Risk and Safety in the IVF Clinic
2. IVF Laboratory Equipment and Culture Systems
8. Essential Instruments and Disposable Supplies for an IVF Laboratory
9. Co2 and Low-O2 Incubators
10. IVF Workstations
11. Culture Media in IVF: Decisions for the Laboratory
3. In Vitro Fertilization
12. Oocyte Denuding
13. Assessment of Oocyte Quality
14. Polarization Microscopy
15. Cumulus Cell Gene Expression in Assessment of Oocyte Quality
4. Embryo Culture Methods
16. Short Culture: Day 1/Day 2/Day 3 Embryo Culture
17. Extended Culture in IVF
18. In Vitro Maturation of Human Oocytes
19. In Vivo Embryo Culture Device
20. Microfluidics for Gamete Manipulation and Embryo Culture
5. Sperm Processing and Selection
21. Sperm Assessment: Traditional Approaches and Their Indicative Value
22. Sperm Assessment: Novel Approaches and Their Indicative Value
23. Sperm Processing for IVF
24. PESA/TESA/TESE Sperm Processing
25. Processing Sperm Samples in HIV-Positive Patients
26. Intracytoplasmic Morphologically Selected Sperm Injection
27. Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories
28. Electrophoretic Sperm Separation
29. Magnetic-Activated Cell Sorting of Human Spermatozoa
30. Polscope-Based Sperm Selection
6. Insemination Procedures
31. Intrauterine Insemination
32. Conventional IVF Insemination
33. Intracytoplasmic Sperm Injection
34. Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization
7. Micromanipulators and Micromanipulation
35. Hydraulic Manipulators for ICSI
36. Research Instruments Micromanipulators
37. Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators
38. The Leica Microsystems’ IMSI System
39. Automated Robotic Intracytoplasmic Sperm Injection
40. Oocyte Treatment and Preparation for Microinjection
41. Livestock Production via Micromanipulation
8. Embryo Evaluation, Grading, and Assisted Hatching
42. Pronuclear Scoring in Human In Vitro Fertilization
43. Cumulative Morphological Assessment of Embryo Quality
44. Metabolomics: The ViaMetrics-E ™ Procedure for Assessing Embryo Viability
45. Oxygen Consumption as an Indicator of Oocyte and Embryo Viability
46. Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection
47. Amino Acid Turnover as a Biomarker of Embryo Viability
48. Real-Time Embryo Monitoring Device for Embryo Selection
49. Assisted Hatching in IVF
9. Biopsy Procedures on Oocytes and Embryos
50. Polar Body Biopsy
51. Cleavage-Stage Embryo Biopsy
52. Embryo Biopsy for PGD: Current Perspective
53. Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects
10. Cryopreservation
54. Sperm Cryopreservation
55. Slow Freezing of Oocytes
56. Vitrification: Research in Animal Models
57. Oocyte Vitrification
58. Slow Freezing of Embryos
59. Vitrification of Embryos
60. Directional Freezing of Reproductive Cells, Tissues, and Organs
61. Ovarian Tissue Cryopreservation
11. Embryo Transfer
62. Assessment of Uterine Receptivity
63. Single Embryo Transfer
64. Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation
65. Cumulus-Aided Embryo Transfer
12. Management and Regulation in the ART Laboratory
66. Data Management in the ART Laboratory: Requirements and Solutions
67. Regulation, Licensing, and Accreditation of the ART Laboratory
68. Legislation in the United Kingdom
69. Regulation, Licensing, and Accreditation of the ART Laboratory in Europe
70. Regulation, Licensing, and Accreditation of ART Laboratories in India
71. Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART
13. Special Topics
72. The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development
73. Nuclear and Cytoplasmic Transfer: Human Applications and Concerns
74. Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization
75. Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence
Backmatter
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Tags: Zsolt Peter Nagy, Alex Varghese, Ashok Agarwal, Practical Manual, Vitro Fertilization