Tumor Immunology Methods and Protocols 1st edition by Attilio Bondanza, Monica Casucci 1493933361‎ 9781493933365

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ISBN 10: 1493933361
ISBN 13: 9781493933365
Author: Attilio Bondanza, Monica Casucci

This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories. This book provides a better understanding of the rules governing tumor and cancer immunology, and discusses innovations in the technology of the immunological “smart bullets” (monoclonal antibodies, vaccines, tumor-reactive T cells) used to specifically target cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Tumor Immunology Methods and Protocols Table of contents:

1. A Regulatory Overview.
2. Natural Antibodies to Tumor
3. Associated Antigens.
4. Generation and Cryopreservation of Clinical Grade Wilms’ Tumor 1 mRNA-Loaded Dendritic Cell Vaccines for Cancer Immunotherapy.
5. Quantitative and Qualitative Analysis of Tumor Associated CD4+ T Cells.
6. Detection and Functional Analysis of Tumor-Derived LXR Ligands.
7. In Vitro Generation of Antigen Specific T Cells from Induced Pluripotent Stem Cells of Antigen Specific T Cell Origin.
8. Chimeric Ant igen Receptors for Cancer Immunotherapy.
9. Cancer and Chemokines.
10. T Cells as Antigen-Carriers for Anti-Tumor Vaccination.
11. Exploiting Secreted Luciferases to Monitor Tumor Progression In Vivo.
12. Efficient RNA Interference of Primary Leukemic Cells for Loss-of-Function Studies in Xenograft Mouse Models.
13. Modeling Human Graft-Versus-Host-Disease in Immunocompromised Mice.
14. ICOS Expression as Immunologic Marker in Immune Activating Monoclonal Antibodies.
15. Identifying NK Alloreactive Donors for Haploidentical Hematopoietic Stem Cell Transplantation.
16. Human Microtumors Generated in 3D: Novel Tools for Integrated In Situ Studies of Cancer Immunotherapies.

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Tags: Tumor Immunology, Methods, Protocols, Attilio Bondanza, Monica Casucci